Biochemical characterization of rice glutelin
WebEurope PMC is an archive of life sciences journal literature. WebThe rice (Oryza sativa L.) mutant of glu4a, lacking the glutelin α-2 subunit while the α-1 subunit increased (α-1H/α-2L), was used in this study. Two-dimensional electrophoresis analysis revealed that the mutant lacked the polypeptide pI6.71/α-2 encoded by glu4 while forming a new polypeptide of pI6.50/α-1. Experiments were conducted to identify the …
Biochemical characterization of rice glutelin
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WebINTRODUCTION. The major endospermic rice protein is glutelin,[1, 2] which comprised up to 70 to 80% of total rice protein.Janick also found 70% of glutelin protein in rice flour as compared to other cereals, which were higher in prolamin contents. The biochemical characterization of rice proteins shows the important nutritional properties and these … WebUnlike other cereals which accumulate prolamins as their primary nitrogen reserve, the major class of storage proteins in rice and oat are the glutelins and 12S globulins, respectively. These proteins are highly homologous at the primary sequence level to the leguminous US globulins. This similarity in protein structure extends to the molecular ...
WebNov 2, 2008 · A new glutelin gene, designated GluD-1, has been discovered by comparing the seed storage proteins from 48 japonica and indica rice cultivars on SDS-PAGE gels. Evidence that GluD-1 is a member of the glutelin family was provided by Western blots using anti-glutelin antiserum and by mapping the gene to the chromosomal glutelin … WebDec 31, 2024 · Enhancing the solubility of rice glutelin in neutral aqueous solution is the prerequisite for the development of rice protein drinks and ingredients. Herein, glutelin …
WebRice glutelin cDNA clones isolated from a rice cDNA library constructed from immature endosperm encoded polypeptides containing the same number of amino acids including a 24 amino acid signal peptide, a 282 amino acid acidic subunit and a 193 amino acid basic subunit. SummaryRice glutelin cDNA clones were isolated from a rice cDNA library … WebJun 15, 2009 · In this study, transgenic a123 expressing glutelin with a mutation at the processing site proved to be useful for studying the glutelin sorting and assembly system in rice seeds. The results obtained using transgenic rice plants clearly address the functional role of post-translational processing from the glutelin precursor to the mature acidic ...
WebJul 1, 2003 · An extensive analysis of the complete rice genome sequence indicates that there are at least 11 glutelin genes (Katsube-Tanaka et al., 2004). However, only GluA …
WebJun 1, 1985 · The two major subunits of rice glutelin, the acidic (alpha) and basic (beta) polypeptides were purified by chromatofocusing and cation … chinatown point best foodWebSep 1, 2012 · The Osborne method comprising of a 2-step in vitro digestion of rice flour was used to extract albumin, globulin, prolamin, and glutelin by using pepsin and trypsin. Subsequently, the antioxidant … Expand gram stain machineWebJun 1, 2003 · L. Q. Qu 81-298-38-8373 81-298-38-8397 [email protected] X. L. Wei H. Satoh T. Kumamaru M. Ogawa F. Takaiwa Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing 100101, China Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan … chinatown point food directoryWebSep 1, 2024 · Rice glutelin has high asparagine and glutamine contents (Liu et al., 2011). Thus, it likely has high ability to form fibril aggregates, since Tang et al. (2010) … chinatown point food 2022WebThe nucleotide sequence revealed that the iso-electric point variation of the pI6.50/α-1 polypeptide was caused by a point mutation with nucleotide replacement at the variable … gram stain ncbiWebNov 2, 2008 · Rice glutelin originates from the same ancestral gene as 11S globulin. Rice glutelin is synthesized as a M r 57 000 (57 kDa) precursor protein and is transported … chinatown point hair salonWebNov 2, 2008 · Abstract. A new glutelin gene, designated GluD-1, has been discovered by comparing the seed storage proteins from 48 japonica and indica rice cultivars on SDS-PAGE gels. Evidence that GluD-1 is a member of the glutelin family was provided by Western blots using anti-glutelin antiserum and by mapping the gene to the … chinatown point chinese restaurant