Phospho erk molecular weight
WebIsotype: Mouse IgG1 Immunogen: Phosphorylated Rat ERK1 (T202/Y204) Peptide Application: Intracellular staining (flow cytometry) (Routinely Tested) Vol. Per Test: 5 µl RRID: AB_10895978 Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide. Regulatory Status: RUO View All Formats Preparation And Storage WebMar 9, 2015 · Finally, pharmacological inhibition of the extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) pathway in α3β1-expressing keratinocytes caused a shift towards long variant expression, while Raf-1-mediated activation of ERK in α3-null keratinocytes dramatically enhanced short variant expression, indicating a role …
Phospho erk molecular weight
Did you know?
WebNov 29, 2024 · Using immunoblot, we detected p-ERK corresponding to its molecular weight in these samples, confirming the integrity of the p-ERK phospho-epitope (Extended Data Fig. 3b). WebThe cells were labeled with ERK1 + ERK2 Antibody [ERK-7D8] (ab54230) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 ® Rabbit Anti-Mouse IgG Secondary Antibody at a dilution of 1/400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI.
WebJan 15, 2024 · Raf. The Raf protein kinase is a protein encoded by the raf gene and is composed of 648 amino acids (aa) with a molecular weight of 40–75 kDa ().Raf exhibits serine/threonine protein kinase activity after binding to Ras. Its molecular structure comprises three conserved regions: Conserved region (CR) l (located at aa 61–194), CR2 … WebFunction: Activation of Erk (extracellular signal regulated kinase) requires the phosphorylation of Thr202 and Tyr204 amino acid residues.. Samples: Jurkat cells were …
WebInvitrogen Anti-Phospho-ERK1/ERK2 (Tyr204) Polyclonal, Catalog # PA5-36038. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and Immunohistochemistry (Paraffin) …
WebNational Center for Biotechnology Information
Web1. block for 1h @ room temperature (RT) with 5% BSA 2. 1:1000 Erk or p-Erk (cell signalling) in 5% BSA, for 1h @ RT and O/N @ 4'C 3. 1:5000 2nd Ab, for 1h @ RT How can I get two … how to start class dojoWebMolecular Weight: 1757.32 Match Criteria: Product Name, Keyword All Photos ( 1) ERK Activation Inhibitor Peptide II, Cell-Permeable - Calbiochem Synonym (s): ERK Activation Inhibitor Peptide II, Cell-Permeable - Calbiochem Empirical Formula (Hill Notation): C136H241N53O31S Molecular Weight: 3146.77 Match Criteria: Product Name, Keyword how to start citationWebThe MEK phosphorylates and activates a mitogen-activated protein kinase (MAPK). RAF and MAPK/ERK are both serine/threonine-specific protein kinases. MEK is a serine/tyrosine/threonine kinase. In a technical sense, … how to start cleaning a messy houseWebWhat is the molecular weight of Erk? Nonphosphorylated p44/42 MAPK (Erk1/2) Control Protein: Bacterially expressed, kinase inactive, Erk2 protein serves as a negative control for Western blotting experiments. Molecular weight of MAP kinase (Erk2) protein is 42 kDa. Supplied in SDS Sample buffer. What is the molecular weight of Erk? What is p44 p42? how to start cleaning a hoarder houseWebKey features and details Rabbit polyclonal to PERK Suitable for: ICC/IF, WB Reacts with: Human Isotype: IgG You may also be interested in Protein Recombinant human PERK protein (ab101115) Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab205718) Primary Anti-PERK antibody [EPR19876-294] (ab229912) View more associated products Overview Product … react cross-envWeb1. block for 1h @ room temperature (RT) with 5% BSA 2. 1:1000 Erk or p-Erk (cell signalling) in 5% BSA, for 1h @ RT and O/N @ 4'C 3. 1:5000 2nd Ab, for 1h @ RT How can I get two bands for... how to start cleaning out foreclosed homesWebSpecificity and sensitivity of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody. The antibody reacts specifically with as little … react crm github