Phospho irf3

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August undefined, 2024

WebThe phospho-IRF3 cell based assay kit conveniently and accurately quantifies phosphorylated IRF3 at Ser386. In viral infected cells, phosphorylation of transcription … WebApr 23, 2024 · We focused on the phosphorylation of IRF3, which is a downstream factor of the cGAS pathway . As shown in Figure 2A, the expression of phosphorylated-IRF3 (p-IRF3) was increased in HNSCC cells after X-ray irradiation, and cGAS knockdown suppressed the radiation-increased p-IRF3. These results suggest that ionizing radiation activates IRF3 ...

Difficulty detecting human phospho-IRF3 on Western Blot

WebJul 7, 2024 · Phospho-S365 acts as a docking site for IRF3, a transcription factor that is phosphorylated and activated by TBK1, leading to transcriptional induction of type I … WebJul 7, 2024 · Phospho-S365 acts as a docking site for IRF3, a transcription factor that is phosphorylated and activated by TBK1, leading to transcriptional induction of type I interferons (IFNs). literary moralist

The cGAS/STING/TBK1/IRF3 innate immunity pathway maintains …

WebThis Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 647 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb #29047. Product Usage Information Storage WebPhospho-IRF3 (S386) and Total IRF3 (S386) ELISA Kit (ab279834) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated IRF3 protein in your experimental model system, you can verify pathway activation in your cell lysates. WebI'm using Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb #29047 from CST to detect phospho-IRF3 using both PMA-differentiated THP-1 and HT-29 cells treated with 2.5ug/mL LMW poly I:C by... importance of tiktok for business

Phospho-IRF3 (S386) and Total IRF3 ELISA Kit (ab279834)

Interferon-independent STING signaling promotes …

WebPhosphorylated IRF3 forms a dimer that translocates to the nucleus and induces type I IFNs and other cytokines. After activation by STING, TBK1 and its homolog IκB kinase epsilon (IKKε) can both lead to activation of the IKK complex, which then activates the transcription factor nuclear factor κB (NF-κB) (11, 12). WebMar 30, 2024 · Similarly, the IRF7, IRF1, and phospho-IRF3 levels hiked after two days of SARS-CoV-2 infection. These findings suggested that IRF7, IRF1, and IRF3 were stimulated following HCoV infections. importance of timber treatmentWebPhospho-IRF3 (S386) ELISA Kit (ab279833) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated IRF3 protein in your experimental model system, you can verify pathway activation in your cell lysates. importance of tillage

"WebInvitrogen Anti-Phospho-IRF3 (Ser385) Polyclonal, Catalog # PA5-36775. Tested in Western Blot (WB) and Immunohistochemistry (Paraffin) (IHC … " - Phospho irf3

Phospho irf3

Web环保商城会员企业上海邦景实业有限公司提供杂交瘤细胞；tbcd6规格, 细胞名称杂交瘤细胞；tbcd6规格形态特性淋巴母细胞样生长特性半贴壁生长特征特性该细胞属保藏，其特征特性尚未公开。培养条件 IRF3 is a member of the interferon regulatory transcription factor (IRF) family. IRF3 was originally discovered as a homolog of IRF1 and IRF2. IRF3 has been further characterized and shown to contain several functional domains including a nuclear export signal, a DNA-binding domain, a C-terminal IRF association domain and several regulatory phosphorylation sites. IRF3 is found …

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WebApr 13, 2024 · Antibodies. The following antibodies were used: mouse monoclonal antibodies to α-tubulin (Santa Cruz, sc23948), GAPDH (Santa Cruz, sc32233), phospho-H2A.X (Ser139) (EMD Millipore, 05-636), anti ... Web55. A method of treating, inhibiting, reducing, ameliorating, and/or preventing a cancer and or metastasis in a subject comprising administering to a subject an adoptive immunothe

WebWestern blot analysis of extracts of NIH/3T3 cells, using Phospho-IRF3-S396 antibody (AP0623) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. WebAll lanes : Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493) at 0.2 µg/ml (purified) Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates Lane 2 : …

Web"Phospho-IRF3 (Ser396) antibodies" comparison At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs. WebPhospho-IRF3 (Ser396) Antibody (BS-3195R) in WB Mouse liver lysates probed with Anti-IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:300 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37°C. Product Details Target Information

Web0.1ml Phospho-IRF3 (Ser396) 磷酸化干扰素调节因子3 130428-200503 0.2ml IRAK4 白介素-1受体相关激酶4抗体 130429-200203 0.1ml phospho-IRAK4(Thr345) 磷酸化白介素-1受体相关激酶4抗体 130430-200305 0.2ml IL-1RA 白介素-1受体拮抗剂抗体 0431-9501

WebJun 1, 2024 · IRF-3 Antibody (SL-12) is a mouse monoclonal IgG 1 κ IRF-3 antibody, cited in 73 publications, provided at 200 µg/ml raised against recombinant IRF-3 fusion protein corresponding to human IRF-3 (amino acids 56-427) IRF-3 Antibody (SL-12) is recommended for detection of IRF-3 of mouse, rat and human origin by WB, IP, IF and IHC … literary moods listWebPhospho-IRF3 (S386) ELISA Kit (ab279833) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human … literary mood vs toneWebJun 10, 2016 · Each plate also contained positive controls (p65, MAVS, IRF3-5D (constitutively active IRF3), IKK-SE (constitutively active IKK) TRIF),negative controls (pcDNA3.1 vector, GFP, pCherryvectors), emptywells. HEK293T/NF-kB-luciferasecell line high-throughputtrans- fection Fugene6transfection reagent (Roche). importance of time essay in marathiWebPhospho-IRF3 (Ser396) Antibody (720012) in ICC/IF. Immunofluorescence was performed on fixed and permeabilized HeLa cells treated with Calyculin A (50 ng/mL/20 min) for … importance of time boundaries in counsellingWebSep 19, 2013 · IRF3 is a constitutively expressed transcription factor that resides in the cytoplasm and dimerizes and translocates to the nucleus upon phosphorylation ( 6 ). … importance of time and space complexityWebWestern Blot using Phospho-IRF3 (Ser386) Polyclonal Antibody ( Product # PA5-78025 ). Untreated (–) and treated (+) THP-1 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Phospho-IRF3 (Ser386) Polyclonal Antibody ( Product # PA5-78025) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody ... importance of time and attendanceWebNational Center for Biotechnology Information importance of tiklos